Determination of quinolone antibiotics by biomass waste-monolithic spin columns-solid phase extraction coupled with liquid chromatography
International Journal of Development Research
Determination of quinolone antibiotics by biomass waste-monolithic spin columns-solid phase extraction coupled with liquid chromatography
Received 12th August, 2021 Received in revised form 15th September, 2021 Accepted 17th October, 2021 Published online 30th November, 2021
Copyright © 2021, Xiangjin Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
A simple, green and rapid method for the simultaneous determination of three kinds of quinolone antibiotics (QNs) from environmental water samples and biological samples was established by monolithic spin columns-solid phase extraction coupled with high performance liquid chromatography (MSC-SPE-HPLC). The QNs was completely adsorbed by 300 mg spent coffee grounds as the sorbents of MSC-SPE at 1000 rpm, and eluted by 2500 μL acetonitrile and 4% acetic acid (60:40, V:V). The efficient and rapid elution was obtained under the speed of 500 rpm. After separation on a Pursuit 5 C18 column (250 mm×4.6 μm, 5 μm), the samples were determined. The three QNs showed good liner relationship in the range of 0.1-10 μg/mL with the correlation coefficients greater than 0.998. The limit of detection (LODs, S/N=3) for ciprofloxacin, norfloxacin and enrofloxacin was 33.3 ng/mL.Moreover, the recoveries were 70.3-112.6% at three spiked levels (0.5, 1 and 2 μg/mL) with the relative standard deviation 1.02-9.78%. The obtained results revealed that the method was simple to operate, green and environment-friendly, and could be used for the determination of antibiotics in environmental samples.
