Genetic variation of 15 autosomal short tandem repeat (str) loci in sample of palestinian population residing in Iraq

International Journal of Development Research

Genetic variation of 15 autosomal short tandem repeat (str) loci in sample of palestinian population residing in Iraq

Abstract: 

Allele frequencies for the 15 autosomal STR loci included in the AmpFlSTR1 Identifiler TM PCR Amplification Kit panel from Applied Biosystems (D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, CSF1PO, D19S433, D2S1338, D16S539) and several statistical parameters were estimated from a sample of 106 unrelated individuals. samples were extracted using a Prep Filer Forensic DNA Extraction Kit (Applied Biosystems, Foster City, CA), DNA quantified using Nano drop Thomson. a different number of alleles were observed with frequencies ranging between 0.005 (D8S1197- allele 9, D21S11- allele 26 and 33, D13S317- allele 15, vWA – allele 11and 20, D18S51 - allele  20and FGA- allele  28) and 0.443 (TPOX-allele 8). No significant departure from Hardy Weinberg Equilibrium (HWE) expectations were observed (a 5% significance level was taken) in the Palestinian Population residing in Iraq. The combined probability of exclusion, power of discrimination, probability of matching value for all the 15 STR loci were 0.99989468; 0.999999 and 1.0597E-18, respectively.

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