Microbiological characterization and antibiotic sensitivity pattern of pseudomonas aeruginosa isolated from clinical samples

Pseudomonas aeruginosa is intrinsically resistant to several antimicrobials and rapidly develop resistance to other drugs during treatment and make it difficult and ineffective. In this study Pseudomonas aeruginosa was isolated and clinically characterized from clinical samples and its antibiotic sensitivity pattern towards commonly prescribed Beta-lactum antibiotic disc. Analysis by Gram staining and culturingon nutrient agar, blood agar and MacConkey agar plates revealed that among the 154 sputum samples, only eight were with Pseudomonas aeruginosa whereas 11 out of 462 urine samples and 13 of 100 pus samples were positive to Pseudomonas aeruginosa. The isolates were identified as Pseudomonas aeruginosa, by clinically standard confirmative protocols. The isolates are motile, Gram negative bacilli, citrate, catalase and oxidase positive, indole and urease negative, triple sugar iron test, Mannitol non-fermentative and motile. Antibiotic sensitivity pattern of the characterized isolates were by Kirby-Bauer disc diffusion method against beta-lactam antibiotic discs revealed that all the Pseudomonas aeruginosa isolates are resistant to antibiotics other than gentamicin. Thus the present study indicates the prevalence of multi drug resistant Pseudomonas aeruginosa strains, among clinically ill cases and implies the necessity of an immediate solution or alternative to overcome any possible Pseudomonas aeruginosa out breaks.